Enzymatic synthesis of a 6-sialyl lactose analogue using a pH-responsive water-soluble polymer support

Bioorg Med Chem Lett. 2011 Sep 1;21(17):5041-4. doi: 10.1016/j.bmcl.2011.04.076. Epub 2011 Apr 24.

Abstract

The Letter describes a strategy for the enzymatic synthesis of glycans based on a pH-responsive water-soluble polymer. In neutral condition, the polymer is water-soluble and convenient for in-solution enzymatic synthesis, whereas in acidic condition (pH lower than 4.0), the polymer disconnects with the product and becomes insoluble, which can be easily removed. A 6-Sialyl lactose analogue was synthesized as a model reaction using this approach.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Hydrogen-Ion Concentration*
  • Lactose / analogs & derivatives*
  • Lactose / chemical synthesis
  • Molecular Sequence Data
  • Polymers / chemistry*
  • Solubility
  • Water / chemistry*

Substances

  • Polymers
  • Water
  • Lactose