Inhibition of the NF-kappaB pathway in human intestinal epithelial cells by commensal Streptococcus salivarius

Appl Environ Microbiol. 2011 Jul;77(13):4681-4. doi: 10.1128/AEM.03021-10. Epub 2011 May 20.

Abstract

Streptococcus salivarius exhibited an anti-inflammatory effect on intestinal epithelial cells (IECs) and monocytes. Strains were screened using a reporter clone, HT-29/kB-luc-E, induced by tumor necrosis factor alpha (TNF-α). Supernatant from each strain downregulated NF-κB activation. The two most efficient strains produced an active metabolite (<3 kDa) which was able to downregulate the secretion of the proinflammatory chemokine interleukin-8 (IL-8).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cytokines / antagonists & inhibitors
  • Epithelial Cells / immunology*
  • Epithelial Cells / microbiology*
  • Genes, Reporter
  • Humans
  • Immunologic Factors / analysis
  • Immunologic Factors / isolation & purification
  • Luciferases / metabolism
  • Molecular Weight
  • NF-kappa B / antagonists & inhibitors*
  • Streptococcus / chemistry
  • Streptococcus / immunology*

Substances

  • Cytokines
  • Immunologic Factors
  • NF-kappa B
  • Luciferases