Abstract
Cells containing a deletion of either the SCR1 or SCR2 genes, which code for the 7SL RNA component of the signal recognition particle (SRP) homologue, were found to be viable. Two independent approaches demonstrated that cells containing deletions of both genes were inviable. Therefore, Yarrowia lipolytica contains two (and only two) functional 7SL RNA genes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Blotting, Southern
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Chromosome Deletion
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Genes, Fungal
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RNA, Fungal / genetics*
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Restriction Mapping
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Ribonucleoproteins / genetics*
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Saccharomycetales / genetics*
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Saccharomycetales / growth & development
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Signal Recognition Particle
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Transformation, Genetic
Substances
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RNA, Fungal
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Ribonucleoproteins
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Signal Recognition Particle