Abstract
An enzyme-magnetic nanoparticle conjugate is prepared via conjugation of Ni(2+) ions onto the surface of magnetic nanoparticles to interact with a six histidine-tagged enzyme. The catalytic properties and enzyme rigidification of the conjugates are more stable at high concentrations of aromatic hydrocarbons.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biocatalysis
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Catechol 1,2-Dioxygenase / chemistry
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Catechol 1,2-Dioxygenase / genetics
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Catechol 1,2-Dioxygenase / metabolism
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Enzymes / chemistry
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Enzymes / genetics
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Enzymes / metabolism*
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Ferrosoferric Oxide / chemistry
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Histidine / genetics
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Histidine / metabolism
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Hydrocarbons, Aromatic / chemistry
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Hydrocarbons, Aromatic / metabolism*
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Magnetite Nanoparticles / chemistry*
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Nickel / chemistry
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Oligopeptides / genetics
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Oligopeptides / metabolism
Substances
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Enzymes
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His-His-His-His-His-His
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Hydrocarbons, Aromatic
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Magnetite Nanoparticles
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Oligopeptides
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Histidine
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Nickel
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Catechol 1,2-Dioxygenase
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Ferrosoferric Oxide