Abstract
Recombinant viruses derived from coronaviruses mouse hepatitis virus strains JHM and A59, were used to map biologic properties of the virus to viral genes. The 3' portion (about 25%) of the viral genome, including the genes coding for all of the structural proteins, controls biologic properties such as organ tropism of the virus, pattern of the virus-induced central nervous system pathology in mice, plaque morphology, and virus yield in tissue culture.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Brain / pathology
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Central Nervous System Diseases / microbiology
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Central Nervous System Diseases / pathology
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Genes, Viral
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Hepatitis, Viral, Animal / pathology
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Liver / pathology
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Mice
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Mice, Inbred C57BL
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Murine hepatitis virus / genetics
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Murine hepatitis virus / pathogenicity*
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Organ Specificity
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RNA, Viral / genetics*
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Recombination, Genetic
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Spleen / pathology
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Thymus Gland / pathology
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Virulence / genetics
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Virus Cultivation