Abstract
The majority of current high-throughput protein purification protocols include rate-limiting centrifugation steps. A column and nozzle assembly was developed that can be used in-line with microfluidization for the purification of bacterially-overexpressed, His-tagged proteins directly from bacterial cultures. Yields and purity are comparable with standard protocols. This large-scale protein purification protocol is easy to use and widely-applicable.
Copyright © 2011 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Centrifugation
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Chromatography, Affinity / methods*
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Cloning, Molecular
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Escherichia coli
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Genetic Vectors
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Histidine / chemistry
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Histidine / metabolism
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Humans
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Microfluidic Analytical Techniques* / instrumentation
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Oligopeptides / chemistry
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Oligopeptides / metabolism
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Plasmids
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / isolation & purification*
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Silicon Dioxide / chemistry
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Silicon Dioxide / metabolism
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Transformation, Bacterial
Substances
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His-His-His-His-His-His
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Oligopeptides
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Recombinant Fusion Proteins
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Histidine
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Silicon Dioxide