A centrifugation-free high-throughput protein purification system using in-line microfluidization

Protein Expr Purif. 2011 Oct;79(2):204-9. doi: 10.1016/j.pep.2011.05.006. Epub 2011 May 14.

Abstract

The majority of current high-throughput protein purification protocols include rate-limiting centrifugation steps. A column and nozzle assembly was developed that can be used in-line with microfluidization for the purification of bacterially-overexpressed, His-tagged proteins directly from bacterial cultures. Yields and purity are comparable with standard protocols. This large-scale protein purification protocol is easy to use and widely-applicable.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Centrifugation
  • Chromatography, Affinity / methods*
  • Cloning, Molecular
  • Escherichia coli
  • Genetic Vectors
  • Histidine / chemistry
  • Histidine / metabolism
  • Humans
  • Microfluidic Analytical Techniques* / instrumentation
  • Oligopeptides / chemistry
  • Oligopeptides / metabolism
  • Plasmids
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / isolation & purification*
  • Silicon Dioxide / chemistry
  • Silicon Dioxide / metabolism
  • Transformation, Bacterial

Substances

  • His-His-His-His-His-His
  • Oligopeptides
  • Recombinant Fusion Proteins
  • Histidine
  • Silicon Dioxide