Development of microsatellite markers for Aulonemia aristulata (Poaceae) and cross-amplification in other bamboo species

Am J Bot. 2011 Apr;98(4):e90-2. doi: 10.3732/ajb.1000511. Epub 2011 Mar 30.

Abstract

Premise of the study: Microsatellite primers were developed for Aulonemia aristulata, an endangered species of economic interest, to further describe its genetic variability and population structure. We also tested cross-amplification in 18 other bamboo species.

Methods and results: Using an enrichment genomic library, 13 microsatellite loci were isolated and characterized in A. aristulata. Seven of these loci were polymorphic. Twelve markers were cross-amplified in at least ten of the tested bamboo species.

Conclusions: These markers will be useful for studies on the genetic diversity and structure of A. aristulata, which are important for future conservation, management and breeding programs of this species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bambusa / genetics
  • DNA Primers*
  • DNA, Plant / analysis*
  • Genetic Loci*
  • Genome, Plant*
  • Genomic Library
  • Microsatellite Repeats*
  • Poaceae / genetics*
  • Polymorphism, Genetic*
  • Species Specificity

Substances

  • DNA Primers
  • DNA, Plant