An enzyme-linked immunosorbent assay for detection of bovine leukaemia virus p24 antibody in cattle

J B Molloy; P J Walker; F C Baldock; B J Rodwell; J A Cowley

doi:10.1016/0166-0934(90)90086-u

An enzyme-linked immunosorbent assay for detection of bovine leukaemia virus p24 antibody in cattle

J Virol Methods. 1990 Apr;28(1):47-57. doi: 10.1016/0166-0934(90)90086-u.

Authors

J B Molloy¹, P J Walker, F C Baldock, B J Rodwell, J A Cowley

Affiliation

¹ Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, Australia.

PMID: 2161419
DOI: 10.1016/0166-0934(90)90086-u

Abstract

An ELISA for detecting antibody to the bovine leukaemia virus (BLV) core protein p24 is described. The test uses p24 antigen purified from concentrated cell culture supernate by lectin-affinity chromatography and gel filtration. The sensitivity and specificity of the p24-ELISA for diagnosing BLV infection relative to the gp51 agar gel immunodiffusion test, were 98.1 and 96.7%, respectively. In the event of widespread use of gp51 based vaccines, the p24-ELISA should differentiate effectively between naturally infected and vaccinated animals.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral / analysis*
Antigens, Viral / immunology
Cattle
Cattle Diseases / diagnosis*
Chromatography, Affinity
Chromatography, Gel
Enzyme-Linked Immunosorbent Assay
Immunoblotting
Leukemia / diagnosis
Leukemia / veterinary*
Leukemia Virus, Bovine / immunology*
Retroviridae / immunology*
Retroviridae Proteins, Oncogenic / immunology*
Viral Core Proteins / immunology*

Substances

Antibodies, Viral
Antigens, Viral
Retroviridae Proteins, Oncogenic
Viral Core Proteins