Abstract
The migration of cortical γ-aminobutyric acidergic interneurons has been extensively studied in rodent embryos, whereas few studies have documented their postnatal migration. Combining in vivo analysis together with time-lapse imaging on cortical slices, we explored the origin and migration of cortical interneurons during the first weeks of postnatal life. Strikingly, we observed that a large pool of GAD65-GFP-positive cells accumulate in the dorsal white matter region during the first postnatal week. Part of these cells divides and expresses the transcription factor paired box 6 indicating the presence of local transient amplifying precursors. The vast majority of these cells are immature interneurons expressing the neuronal marker doublecortin and partly the calcium-binding protein calretinin. Time-lapse imaging reveals that GAD65-GFP-positive neurons migrate from the white matter pool into the overlying anterior cingulate cortex (aCC). Some interneurons in the postnatal aCC express the same immature neuronal markers suggesting ongoing migration of calretinin-positive interneurons. Finally, bromodeoxyuridine incorporation experiments confirm that a small fraction of interneurons located in the aCC are generated during the early postnatal period. These results altogether reveal that at postnatal ages, the dorsal white matter contains a pool of interneuron precursors that divide and migrate into the aCC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Age Factors
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Animals
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Animals, Newborn
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Bromodeoxyuridine / metabolism
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Cell Movement / genetics
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Cell Proliferation
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Cerebral Cortex / cytology*
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Cerebral Cortex / growth & development*
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Embryo, Mammalian
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Eye Proteins
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Female
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GABAergic Neurons / physiology*
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Gene Expression Regulation, Developmental / genetics
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Gene Expression Regulation, Developmental / physiology*
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Glutamate Decarboxylase / genetics
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Glutamate Decarboxylase / metabolism
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Homeodomain Proteins
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In Vitro Techniques
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Interneurons / physiology*
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Ki-67 Antigen / genetics
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Ki-67 Antigen / metabolism
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Nerve Fibers, Myelinated / metabolism
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Nerve Fibers, Myelinated / physiology*
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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PAX6 Transcription Factor
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Paired Box Transcription Factors
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Pregnancy
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Proteins / genetics
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RNA, Untranslated
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Receptors, Serotonin, 5-HT3 / genetics
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Repressor Proteins
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Thyroid Nuclear Factor 1
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Eye Proteins
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Gt(ROSA)26Sor non-coding RNA, mouse
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Homeodomain Proteins
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Ki-67 Antigen
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Luminescent Proteins
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Nerve Tissue Proteins
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Nuclear Proteins
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PAX6 Transcription Factor
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Paired Box Transcription Factors
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Pax6 protein, mouse
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Proteins
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RNA, Untranslated
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Receptors, Serotonin, 5-HT3
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Repressor Proteins
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Thyroid Nuclear Factor 1
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Transcription Factors
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Glutamate Decarboxylase
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glutamate decarboxylase 2
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Bromodeoxyuridine