Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy

Chem Commun (Camb). 2011 Jul 14;47(26):7368-70. doi: 10.1039/c1cc11893e. Epub 2011 May 27.

Abstract

Attachment of two nitrilotriacetic acid-based ligands to a protein α-helix in an i, i + 4 configuration produces an octadentate chelating motif that is able to bind paramagnetic lanthanide ions rigidly and with high affinity, leading to large pseudocontact shifts and residual dipolar couplings in the NMR spectrum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Chelating Agents / chemistry*
  • Humans
  • Lanthanoid Series Elements / metabolism*
  • Models, Molecular
  • Nitrilotriacetic Acid / chemistry*
  • Nuclear Magnetic Resonance, Biomolecular*
  • Protein Binding
  • Protein Engineering / methods*
  • Ubiquitin / chemistry*
  • Ubiquitin / genetics
  • Ubiquitin / metabolism*

Substances

  • Chelating Agents
  • Lanthanoid Series Elements
  • Ubiquitin
  • Nitrilotriacetic Acid