Abstract
Recent advances in cell biology have demonstrated the role of multiple signaling proteins in the transduction of external signals to cytoplasmic and nuclear effectors, controlling the movement and/or retention of hematopoietic stem cells and progenitors (HSC/P) within the bone marrow, with important clinical implications. Multiple assays have become routine in the analysis of adhesion to the microenvironment, migration toward chemoattractant gradients, and homing of HSC/P in the bone marrow in vivo. In this chapter, we analyze some of the most frequently used assays in our laboratory to explore the ability of HSC to migrate, adhere, and home in in vitro and in vivo assays.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Bone Marrow / physiology
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Cell Adhesion / physiology*
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Cell Movement / physiology*
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Chemokine CXCL12 / genetics
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Chemokine CXCL12 / metabolism*
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Chemotaxis / physiology
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Colony-Forming Units Assay
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Female
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Fibronectins / genetics
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Fibronectins / metabolism*
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Flow Cytometry
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Hematopoietic Stem Cell Mobilization
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Hematopoietic Stem Cell Transplantation
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Hematopoietic Stem Cells* / cytology
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Hematopoietic Stem Cells* / physiology
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Humans
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Mice
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Mice, Inbred C57BL
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Signal Transduction / physiology*
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Whole-Body Irradiation
Substances
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Chemokine CXCL12
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Cxcl12 protein, mouse
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Fibronectins
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Recombinant Proteins