The earliest intrathymic precursors of CD8α(+) thymic dendritic cells correspond to myeloid-type double-negative 1c cells

Eur J Immunol. 2011 Aug;41(8):2165-75. doi: 10.1002/eji.201141728. Epub 2011 Jul 4.

Abstract

The dendritic cells (DCs) present in lymphoid and non-lymphoid organs are generated from progenitors with myeloid-restricted potential. However, in the thymus a major subset of DCs expressing CD8α and langerin (CD207) appears to stand apart from all other DCs in that it is thought to derive from progenitors with lymphoid potential. Using mice expressing a fluorescent reporter and a diphtheria toxin receptor under the control of the cd207 gene, we demonstrated that CD207(+) CD8α(+) thymic DCs do not share a common origin with T cells but originate from intrathymic precursors that express markers that are normally present on all (CD11c(+) and MHCII molecules) or on some (CD207, CD135, CD8α, CX3CR1) DC subsets. Those intrathymic myeloid-type precursors correspond to CD44(+) CD25(-) double-negative 1c (DN1c) cells and are continuously renewed from bone marrow-derived canonical DC precursors. In conclusion, our results demonstrate that the earliest intrathymic precursors of CD8α(+) thymic DCs correspond to myeloid-type DN1c cells and support the view that under physiological conditions myeloid-restricted progenitors generate the whole constellation of DCs present in the body including the thymus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / genetics
  • Antigens, Surface / immunology
  • Antigens, Surface / metabolism
  • CD8 Antigens / immunology*
  • CD8 Antigens / metabolism
  • Cell Differentiation / immunology
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism
  • Female
  • Flow Cytometry
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Hyaluronan Receptors / immunology
  • Hyaluronan Receptors / metabolism
  • Interferon Regulatory Factors / immunology
  • Interferon Regulatory Factors / metabolism
  • Interleukin-2 Receptor alpha Subunit / immunology
  • Interleukin-2 Receptor alpha Subunit / metabolism
  • Lectins, C-Type / genetics
  • Lectins, C-Type / immunology
  • Lectins, C-Type / metabolism
  • Male
  • Mannose-Binding Lectins / genetics
  • Mannose-Binding Lectins / immunology
  • Mannose-Binding Lectins / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Models, Immunological
  • Myeloid Cells / immunology*
  • Myeloid Cells / metabolism
  • Spleen / cytology
  • Spleen / immunology
  • Spleen / metabolism
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism
  • Thymus Gland / cytology
  • Thymus Gland / immunology*
  • Thymus Gland / metabolism
  • Time Factors

Substances

  • Antigens, Surface
  • CD8 Antigens
  • CD8alpha antigen
  • Cd207 protein, mouse
  • Hyaluronan Receptors
  • Interferon Regulatory Factors
  • Interleukin-2 Receptor alpha Subunit
  • Lectins, C-Type
  • Mannose-Binding Lectins
  • enhanced green fluorescent protein
  • interferon regulatory factor-8
  • Green Fluorescent Proteins