Angiography in the isolated perfused kidney: radiological evaluation of vascular protection in tissue ablation by nonthermal irreversible electroporation

Cardiovasc Intervent Radiol. 2012 Apr;35(2):383-90. doi: 10.1007/s00270-011-0187-x. Epub 2011 Jun 2.

Abstract

Purpose: The nonthermal irreversible electroporation (NTIRE) is a novel nonthermal tissue ablation technique by local application of high-voltage current within microseconds leading to a delayed apoptosis. The purpose of this experimental study was the first angiographic evaluation of the acute damage of renal vascular structure in NTIRE.

Methods: Results of conventional dynamic digital substraction angiography (DSA) and visualization of the terminal vascular bed of renal parenchyma by high-resolution X-ray in mammography technique were evaluated before, during, and after NTIRE of three isolated perfused porcine ex vivo kidneys.

Results: In the dedicated investigation, no acute vascular destruction of the renal parenchyma and no dysfunction of the kidney perfusion model were observed during or after NTIRE. Conspicuous were concentric wave-like fluctuations of the DSA contrast agent simultaneous to the NTIRE pulses resulting from NTIRE pulse shock wave.

Conclusion: The NTIRE offers an ablation method with no acute collateral vascular damage in angiographic evaluation.

MeSH terms

  • Angiography, Digital Subtraction
  • Animals
  • Electroporation / methods*
  • In Vitro Techniques
  • Kidney / blood supply*
  • Kidney / diagnostic imaging*
  • Perfusion
  • Swine