Abstract
The brain and dendritic cell (BAD)-associated lysosome-associated membrane protein (LAMP)-like molecule (BAD-LAMP, c20orf103, UNC-46) is a newly identified member of the family of LAMPs. BAD-LAMP expression in the mouse is confined to neurons. We demonstrate here that in humans, BAD-LAMP can specifically be found in the type I IFN-producing plasmacytoid dendritic cells (pDCs). Human BAD-LAMP is localized in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of freshly isolated CD123(+) pDCs and is rapidly lost upon activation by unmethylated cytosine-phosphate-guanine (CpG) oligonucleotides. The restricted pattern of BAD-LAMP expression allows for the rapid identification of normal and leukemic human pDCs in tissues and blood.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomarkers / metabolism*
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Brain / metabolism
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Dendritic Cells / cytology
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Dendritic Cells / metabolism*
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Endoplasmic Reticulum / metabolism
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Endosomes / metabolism
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Golgi Apparatus / metabolism
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HeLa Cells
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Humans
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Interleukin-3 / metabolism
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Interleukin-3 Receptor alpha Subunit / metabolism
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Leukemia / metabolism
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Leukemia / pathology*
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Lysosomal Membrane Proteins / genetics
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Lysosomal Membrane Proteins / metabolism*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism
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Oligonucleotide Array Sequence Analysis
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Oligonucleotides / genetics
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Oligonucleotides / metabolism
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Transfection
Substances
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Biomarkers
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IL3RA protein, human
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Interleukin-3
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Interleukin-3 Receptor alpha Subunit
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LAMP5 protein, human
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Lysosomal Membrane Proteins
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Membrane Proteins
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Membrane Transport Proteins
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Oligonucleotides
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UNC93B1 protein, human