Successful extraction of DNA from archived alcohol-fixed white-eye fish specimens using an ancient DNA protocol

J Fish Biol. 2011 Jun;78(7):2074-9. doi: 10.1111/j.1095-8649.2011.02975.x. Epub 2011 May 10.

Abstract

A protocol used routinely for rapid ancient DNA extraction was applied to fish tissue archived over 80 years ago. The method proved successful, whereas other extraction protocols failed. Researchers working on DNA from older archived fish samples are encouraged to continue to concentrate their efforts on 'white-eye' specimens, which indicate an alcohol-based fixative and are thus likely to yield viable DNA.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Beloniformes / genetics*
  • Conservation of Natural Resources / methods
  • Cyprinodontiformes / genetics*
  • DNA / chemistry
  • DNA / isolation & purification*
  • Fixatives / chemistry
  • Genetic Techniques

Substances

  • Fixatives
  • DNA