Rapid titration of viruses by flow cytometry

Curr Protoc Cell Biol. 2011 Jun:Chapter 26:Unit 26.11. doi: 10.1002/0471143030.cb2611s51.

Abstract

Traditionally, the most common methods used to titrate virus stocks are the plaque assay and the hemagglutination assay. The protocol presented here is based on the detection of viral-expressed proteins in infected cells by flow cytometry. It is simpler and more rapid than the traditional plaque-forming assay and it enables high-throughput analyses.

MeSH terms

  • Animals
  • Antibodies, Viral / analysis
  • Cell Line
  • Flow Cytometry / methods*
  • Simian virus 40 / isolation & purification
  • Viral Load / methods*
  • Viruses / isolation & purification*

Substances

  • Antibodies, Viral