Abstract
The rules of nucleic acid base-pairing have been used to construct nanoscale architectures and organize biomolecules, but little has been done to apply this technology in vivo. We designed and assembled multidimensional RNA structures and used them as scaffolds for the spatial organization of bacterial metabolism. Engineered RNA modules were assembled into discrete, one-dimensional, and two-dimensional scaffolds with distinct protein-docking sites and used to control the spatial organization of a hydrogen-producing pathway. We increased hydrogen output as a function of scaffold architecture. Rationally designed RNA assemblies can thus be used to construct functional architectures in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aptamers, Nucleotide / chemistry
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Aptamers, Nucleotide / metabolism
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Biosynthetic Pathways*
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Escherichia coli / growth & development
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Escherichia coli / metabolism*
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Ferredoxins / chemistry
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Ferredoxins / metabolism*
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Hydrogen / metabolism*
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Hydrogenase / chemistry
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Hydrogenase / metabolism*
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Iron-Sulfur Proteins / chemistry
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Iron-Sulfur Proteins / metabolism*
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Microscopy, Atomic Force
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Nanostructures
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Protein Binding
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Protein Conformation
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RNA / chemistry*
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RNA / metabolism*
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Synthetic Biology / methods
Substances
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Aptamers, Nucleotide
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Ferredoxins
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Iron-Sulfur Proteins
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Recombinant Fusion Proteins
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RNA
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Hydrogen
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iron hydrogenase
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Hydrogenase