Abstract
CD83, a maturation marker for human and mouse dendritic cells (DCs), plays a critical role in CD4(+) T cell development as well as peripheral immune regulation. Here, two novel mouse anti-human CD83 monoclonal antibodies (MAbs) were prepared and their immunological characteristics were determined. Among the two MAbs, 8B4 binds to a linear epitope whereas 1E11 recognizes a conformational epitope. Cross-linking of 8B4 but not 1E11 with CD83-Ig augments the fusion protein mediated inhibition of peripheral blood mononuclear cells (PBMCs). Thus the two MAbs may be good candidates for immunoassaying and functional exploration of CD83 molecule.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal, Murine-Derived / biosynthesis*
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Antibodies, Monoclonal, Murine-Derived / immunology*
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Antibodies, Monoclonal, Murine-Derived / metabolism
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Antigens, CD / genetics
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Antigens, CD / immunology*
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CD83 Antigen
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Cell Line
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Cloning, Molecular
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DNA Primers / genetics
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DNA, Complementary / genetics
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Epitopes, T-Lymphocyte / metabolism
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Humans
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Immunoglobulins / genetics
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Immunoglobulins / immunology*
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Leukocytes, Mononuclear / immunology
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology*
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Mice
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Antibodies, Monoclonal, Murine-Derived
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Antigens, CD
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DNA Primers
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DNA, Complementary
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Epitopes, T-Lymphocyte
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Immunoglobulins
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Membrane Glycoproteins