Enhanced refoldability and thermoactivity of fluorinated phosphotriesterase

Chembiochem. 2011 Aug 16;12(12):1845-8. doi: 10.1002/cbic.201100221. Epub 2011 Jun 27.
No abstract available

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acids / chemistry
  • Amino Acids / metabolism
  • Calorimetry, Differential Scanning
  • Circular Dichroism
  • Cloning, Molecular
  • Dimerization
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme Stability
  • Escherichia coli
  • Escherichia coli Proteins / chemistry*
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism
  • Halogenation
  • Hot Temperature
  • Insecticides / metabolism*
  • Kinetics
  • Models, Molecular
  • Paraoxon / metabolism
  • Phosphoric Triester Hydrolases / chemistry*
  • Phosphoric Triester Hydrolases / genetics
  • Phosphoric Triester Hydrolases / metabolism
  • Protein Engineering / methods*
  • Protein Folding
  • Recombinant Proteins / chemistry*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Substrate Specificity

Substances

  • Amino Acids
  • Escherichia coli Proteins
  • Insecticides
  • Recombinant Proteins
  • Phosphoric Triester Hydrolases
  • Paraoxon