Two distinct types of Fc receptor for IgG on guinea pig macrophages activate the NADPH oxidase through different signal-transduction pathways

Biochem Biophys Res Commun. 1990 Oct 15;172(1):223-8. doi: 10.1016/s0006-291x(05)80197-4.

Abstract

Neither 5 microM calmodulin antagonist W-7 nor depletion of the intracellular Ca2+ affected the O2- generation triggered by the Fc receptor for IgG2 (Fc gamma 2 R) on guinea pig macrophages, although that by the Fc receptor for both IgG1 and IgG2 (Fc gamma 1/gamma 2 R) was lowered to 50% of a control level by W-7 and the Ca2+ depletion, respectively. Staurosporine inhibited all these responses, but to different extents. These results reveal that the signal-transduction pathways linked to these receptors differ from each other, and that Fc gamma 1/gamma 2 R triggers the O2- generation through the Ca2(+)-calmodulin system and the Ca2(+)-independent system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Differentiation / physiology*
  • Calmodulin / antagonists & inhibitors
  • Guinea Pigs
  • Immunoglobulin G / immunology
  • In Vitro Techniques
  • Kinetics
  • Macrophages / drug effects
  • Macrophages / immunology
  • Macrophages / physiology*
  • NADH, NADPH Oxidoreductases / metabolism*
  • NADPH Oxidases
  • Protein Kinase C / antagonists & inhibitors
  • Receptors, Fc / physiology*
  • Receptors, IgG
  • Signal Transduction*
  • Sulfonamides / pharmacology
  • Superoxides / metabolism
  • Tetradecanoylphorbol Acetate / pharmacology

Substances

  • Antigens, Differentiation
  • Calmodulin
  • Immunoglobulin G
  • Receptors, Fc
  • Receptors, IgG
  • Sulfonamides
  • Superoxides
  • W 7
  • NADH, NADPH Oxidoreductases
  • NADPH Oxidases
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate