Studies on chemical fingerprints of Siraitia grosvenorii fruits (Luo Han Guo) by HPLC

J Nat Med. 2012 Jan;66(1):70-6. doi: 10.1007/s11418-011-0555-5. Epub 2011 Jun 30.

Abstract

A novel, efficient, and accurate fingerprinting method using high performance liquid chromatography-photodiode array detection has been developed and optimized for the investigation and demonstration of the variance in chemical properties among Siraitia grosvenorii fruits from different origins. The effects of growth stages, cultivated varieties, collection locations, and fruit portions of the herb on chromatographic fingerprints were examined. Eleven compounds were identified on chromatograms by comparing the retention time and UV spectrum of each peak separately with those of external references. The results revealed that chromatographic fingerprints, combining similarity or hierarchical clustering analysis along with reference compounds, could efficiently identify and distinguish S. grosvenorii fruits from different sources, which provided helpful clues for studying the plants' secondary metabolites and benefitted quality control.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid* / standards
  • Cluster Analysis
  • Cucurbitaceae / chemistry*
  • Cucurbitaceae / growth & development
  • Drugs, Chinese Herbal / analysis*
  • Drugs, Chinese Herbal / isolation & purification
  • Drugs, Chinese Herbal / standards
  • Fruit
  • Plants, Medicinal
  • Quality Control
  • Reference Standards
  • Spectrophotometry, Ultraviolet

Substances

  • Drugs, Chinese Herbal