Abstract
A cell-free preparation obtained from 0- to 5-h-old Drosophila melanogaster embryos induces chromatin decondensation and nuclear formation from demembranated Xenopus sperm. Newly formed nuclei have a peripheral lamina, a double membrane, and structures resembling pore complexes. Indirect immunofluorescence analyses demonstrate the association of Drosophila lamins and DNA topoisomerase II with newly assembled nuclei.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Cell Nucleus* / enzymology
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Cell Nucleus* / metabolism
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Cell Nucleus* / ultrastructure
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Cell-Free System
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Chromatin
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DNA Topoisomerases, Type II / metabolism
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Drosophila melanogaster
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Embryo, Nonmammalian
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Fluorescent Antibody Technique
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In Vitro Techniques
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Lamins
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Male
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Nuclear Envelope / metabolism
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Nuclear Envelope / ultrastructure
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Nuclear Proteins / metabolism
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Spermatozoa / ultrastructure
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Xenopus
Substances
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Antibodies, Monoclonal
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Chromatin
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Lamins
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Nuclear Proteins
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DNA Topoisomerases, Type II