Comparison of the host specificities of two bacteroidales quantitative PCR assays used for tracking human fecal contamination

Laurie C Van De Werfhorst; Bram Sercu; Patricia A Holden

doi:10.1128/AEM.00239-11

Comparison of the host specificities of two bacteroidales quantitative PCR assays used for tracking human fecal contamination

Appl Environ Microbiol. 2011 Sep;77(17):6258-60. doi: 10.1128/AEM.00239-11. Epub 2011 Jul 8.

Authors

Laurie C Van De Werfhorst¹, Bram Sercu, Patricia A Holden

Affiliation

¹ Donald Bren School of Environmental Science and Management, University of California, Santa Barbara, CA 93106-5131, USA. [email protected]

Abstract

The sewage-associated real-time quantitative PCR (qPCR) assays BacHum and HF183 SYBR were compared for specificity against local fecal sources. Both assays were equally sensitive to sewage, but BacHum showed substantially more false-positive results for cat, dog, gull, and raccoon feces.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacteriological Techniques / methods*
Bacteroidetes / genetics*
Bacteroidetes / isolation & purification*
Cats
Charadriiformes
Dogs
False Positive Reactions
Feces / microbiology*
Humans
Polymerase Chain Reaction / methods*
Raccoons
Sensitivity and Specificity