Rapid discrimination between Candida glabrata, Candida nivariensis, and Candida bracarensis by use of a singleplex PCR

J Clin Microbiol. 2011 Sep;49(9):3375-9. doi: 10.1128/JCM.00688-11. Epub 2011 Jul 13.

Abstract

We report here a PCR-based assay using a single primer pair targeting the RPL31 gene that allows discrimination between Candida glabrata, Candida bracarensis, and Candida nivariensis according to the size of the generated amplicon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Candida / classification*
  • Candida / genetics
  • Candida / isolation & purification*
  • Candidiasis / microbiology*
  • DNA Primers / genetics
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Humans
  • Microbiological Techniques / methods*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • DNA Primers
  • DNA, Fungal
  • DNA, Ribosomal Spacer

Associated data

  • GENBANK/JF690246
  • GENBANK/JF690247
  • GENBANK/JN088223