Abstract
In the nematode Caenorhabditis elegans, temperature-sensitive mutants of emb-1 arrest as one-cell embryos in metaphase of meiosis I in a manner that is indistinguishable from embryos that have been depleted of known subunits of the anaphase-promoting complex or cyclosome (APC/C). Here we show that the emb-1 phenotype is enhanced in double mutant combinations with known APC/C subunits and suppressed in double mutant combinations with known APC/C suppressors. In addition to its meiotic function, emb-1 is required for mitotic proliferation of the germline. These studies reveal that emb-1 encodes K10D2.4, a homolog of the small, recently discovered APC/C subunit, APC16.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alleles
-
Amino Acid Sequence
-
Anaphase-Promoting Complex-Cyclosome
-
Animals
-
Base Sequence
-
Caenorhabditis elegans / genetics*
-
Caenorhabditis elegans Proteins / genetics*
-
Cell Proliferation
-
Drosophila Proteins
-
Female
-
Genetic Complementation Test
-
Germ Cells / cytology
-
Germ Cells / metabolism
-
Male
-
Meiosis / genetics
-
Molecular Sequence Data
-
Mutation
-
Phenotype
-
Sequence Homology, Amino Acid
-
Tumor Suppressor Proteins
-
Ubiquitin-Protein Ligase Complexes / genetics*
Substances
-
APC2 protein, Drosophila
-
Caenorhabditis elegans Proteins
-
Drosophila Proteins
-
Tumor Suppressor Proteins
-
emb-1 protein, C elegans
-
Ubiquitin-Protein Ligase Complexes
-
Anaphase-Promoting Complex-Cyclosome