Comparison of a commercially available repetitive-element PCR system (DiversiLab) with PCR ribotyping for typing of clostridium difficile strains

C Eckert; J Van Broeck; P Spigaglia; B Burghoffer; M Delmée; P Mastrantonio; F Barbut

doi:10.1128/JCM.00324-11

Comparison of a commercially available repetitive-element PCR system (DiversiLab) with PCR ribotyping for typing of clostridium difficile strains

J Clin Microbiol. 2011 Sep;49(9):3352-4. doi: 10.1128/JCM.00324-11. Epub 2011 Jul 20.

Authors

C Eckert¹, J Van Broeck, P Spigaglia, B Burghoffer, M Delmée, P Mastrantonio, F Barbut

Affiliation

¹ ER8, Université Pierre et Marie Curie, Paris, France. [email protected]

Abstract

This study compared a repetitive-element PCR (rep-PCR) method (DiversiLab system) to PCR ribotyping. The discriminatory power of rep-PCR was 0.997. Among the PCR ribotype 027 isolates tested, different rep types could be distinguished. rep-PCR showed a higher discriminatory power than PCR ribotyping. Nevertheless, this method requires technical skill, and visual interpretation of rep-PCR fingerprint patterns may be difficult.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Adult
Clostridioides difficile / classification*
Clostridioides difficile / genetics
Humans
Molecular Typing / methods*
Polymerase Chain Reaction / methods*
Sensitivity and Specificity