Diazirine based photoaffinity labeling

Luba Dubinsky; Bastiaan P Krom; Michael M Meijler

doi:10.1016/j.bmc.2011.06.066

Diazirine based photoaffinity labeling

Bioorg Med Chem. 2012 Jan 15;20(2):554-70. doi: 10.1016/j.bmc.2011.06.066. Epub 2011 Jun 29.

Authors

Luba Dubinsky¹, Bastiaan P Krom, Michael M Meijler

Affiliation

¹ Department of Chemistry and National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, Israel.

PMID: 21778062
DOI: 10.1016/j.bmc.2011.06.066

Abstract

Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Diazomethane / chemical synthesis
Diazomethane / chemistry*
Enzymes / chemistry
Enzymes / metabolism
Nucleic Acids / chemistry
Pharmaceutical Preparations / chemistry
Photoaffinity Labels / chemistry*
Protein Interaction Mapping
Proteins / chemistry
Proteins / metabolism

Substances

Enzymes
Nucleic Acids
Pharmaceutical Preparations
Photoaffinity Labels
Proteins
Diazomethane