Abstract
Oligonucleotide hybridization was used as a cell-labeling method to significantly amplify the loading of magnetic probes onto target cells. The method utilized short oligonucleotides as the binding agents between antibodies and superparamagnetic iron oxide. This method not only enabled multiplexed analysis, but also allowed detection of multiple markers on a single sample containing only scant cell numbers.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antibodies, Monoclonal / metabolism
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Cell Line, Tumor
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DNA Probes / chemistry
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DNA Probes / genetics
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DNA Probes / metabolism
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Humans
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Magnetic Phenomena*
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Nucleic Acid Hybridization
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Oligonucleotides / chemistry
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Oligonucleotides / genetics*
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Oligonucleotides / metabolism
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Spectrometry, Fluorescence
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Staining and Labeling / methods*
Substances
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Antibodies, Monoclonal
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DNA Probes
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Oligonucleotides