Anthocyanins isolated from the purple-fleshed sweet potato attenuate the proliferation of hepatic stellate cells by blocking the PDGF receptor

Jae Ho Choi; Yong Pil Hwang; Bong Hwan Park; Chul Yung Choi; Young Chul Chung; Hye Gwang Jeong

doi:10.1016/j.etap.2010.10.011

Anthocyanins isolated from the purple-fleshed sweet potato attenuate the proliferation of hepatic stellate cells by blocking the PDGF receptor

Environ Toxicol Pharmacol. 2011 Jan;31(1):212-9. doi: 10.1016/j.etap.2010.10.011. Epub 2010 Nov 9.

Authors

Jae Ho Choi¹, Yong Pil Hwang, Bong Hwan Park, Chul Yung Choi, Young Chul Chung, Hye Gwang Jeong

Affiliation

¹ Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.

PMID: 21787688
DOI: 10.1016/j.etap.2010.10.011

Abstract

During the process of liver fibrosis, hepatic stellate cells (HSCs) play a critical role in the increased formation and reduced degradation of extracellular matrix in the liver. We investigated the anti-proliferative effects of an anthocyanin fraction (AF), isolated from the purple-fleshed sweet potato, on platelet-derived growth factor (PDGF)-BB-dependent signaling pathways in HSC-T6 cells. HSC proliferation plays a pivotal role in liver fibrogenesis. The AF suppressed HSC activation, including PDGF-induced proliferation and α-smooth muscle actin (α-SMA) expression. Additionally, AF inhibited PDGF-BB-induced Akt and ERK1/2 phosphorylation. AF inhibited the phosphorylation level of PDGF receptor-β (PDGFR-β) following PDGF-BB stimulation, providing a mechanism for the inhibition of AF-mediated kinase. These results suggest that AF suppresses HSC proliferation by blocking PDGFR-β signaling, inhibiting Akt and ERK1/2 activation and α-SMA expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / biosynthesis
Animals
Anthocyanins / isolation & purification
Anthocyanins / pharmacology*
Blotting, Western
Cell Proliferation / drug effects*
Cell Survival / drug effects
Coloring Agents
Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors
Extracellular Signal-Regulated MAP Kinases / physiology
Hepatic Stellate Cells / drug effects*
Ipomoea batatas / chemistry*
L-Lactate Dehydrogenase / metabolism
Oncogene Protein v-akt / antagonists & inhibitors
Oncogene Protein v-akt / physiology
Platelet-Derived Growth Factor / pharmacology
Rats
Receptors, Platelet-Derived Growth Factor / antagonists & inhibitors*
Signal Transduction / drug effects
Tetrazolium Salts
Thiazoles

Substances

Actins
Anthocyanins
Coloring Agents
Platelet-Derived Growth Factor
Tetrazolium Salts
Thiazoles
smooth muscle actin, rat
L-Lactate Dehydrogenase
Receptors, Platelet-Derived Growth Factor
Oncogene Protein v-akt
Extracellular Signal-Regulated MAP Kinases
thiazolyl blue