Salivary gland transcriptome analysis in response to sugar feeding in malaria vector Anopheles stephensi

J Insect Physiol. 2011 Oct;57(10):1399-406. doi: 10.1016/j.jinsphys.2011.07.007. Epub 2011 Jul 20.

Abstract

In this study, we analyzed a small scale transcriptome of salivary glands in sugar fed female mosquitoes. Thirty five percent of the transcripts could not be assigned a function. Some of them may code for salivary gland specific products involved in sugar feeding. We identified and characterized two new putative cDNAs encoding a sugar transporter and a cAMP generating DAPIT (Diabetes-Associated proteins in insulin sensitive tissues). Down regulation of these two cDNAs in response to blood feeding suggest that both AsST and AsDAPIT salivary genes may specifically be involved in the facilitation of sugar metabolism and energy production. The inability to absorb or digest sugar may cause organ failure, improper functioning of nervous system, behavioral disorder and death. Further functional characterization of theses putative transcripts is under investigation to examine their role in the mosquito salivary glands.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anopheles / genetics
  • Anopheles / metabolism*
  • Carbohydrates
  • Feeding Behavior
  • Female
  • Gene Expression Profiling
  • Gene Library
  • Genes, Insect
  • Insect Proteins / genetics
  • Insect Proteins / metabolism*
  • Insect Vectors / genetics
  • Insect Vectors / metabolism*
  • Malaria / transmission
  • Molecular Sequence Data
  • Salivary Glands / metabolism
  • Sequence Analysis, DNA

Substances

  • Carbohydrates
  • Insect Proteins