Mutation analysis of the tyrosine phosphatase PTPN2 in Hodgkin's lymphoma and T-cell non-Hodgkin's lymphoma

Haematologica. 2011 Nov;96(11):1723-7. doi: 10.3324/haematol.2011.041921. Epub 2011 Jul 26.

Abstract

We recently reported deletion of the protein tyrosine phosphatase gene PTPN2 in T-cell acute lymphoblastic leukemia. Functional analyses confirmed that PTPN2 acts as classical tumor suppressor repressing the proliferation of T cells, in part through inhibition of JAK/STAT signaling. We investigated the expression of PTPN2 in leukemia as well as lymphoma cell lines. We identified bi-allelic inactivation of PTPN2 in the Hodgkin's lymphoma cell line SUP-HD1 which was associated with activation of the JAK/STAT pathway. Subsequent sequence analysis of Hodgkin's lymphoma and T-cell non-Hodgkin's lymphoma identified bi-allelic inactivation of PTPN2 in 2 out of 39 cases of peripheral T-cell lymphoma not otherwise specified, but not in Hodgkin's lymphoma. These results, together with our own data on T-cell acute lymphoblastic leukemia, demonstrate that PTPN2 is a tumor suppressor gene in T-cell malignancies.

Publication types

  • Clinical Trial
  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • DNA Mutational Analysis
  • Female
  • Hodgkin Disease* / enzymology
  • Hodgkin Disease* / genetics
  • Humans
  • Janus Kinases / genetics
  • Janus Kinases / metabolism
  • Male
  • Mutation*
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma* / enzymology
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma* / genetics
  • Protein Tyrosine Phosphatase, Non-Receptor Type 2* / genetics
  • Protein Tyrosine Phosphatase, Non-Receptor Type 2* / metabolism
  • STAT Transcription Factors / genetics
  • STAT Transcription Factors / metabolism
  • Signal Transduction / genetics
  • Tumor Suppressor Proteins* / genetics
  • Tumor Suppressor Proteins* / metabolism

Substances

  • STAT Transcription Factors
  • Tumor Suppressor Proteins
  • Janus Kinases
  • PTPN2 protein, human
  • Protein Tyrosine Phosphatase, Non-Receptor Type 2