Abstract
PMLA nanoparticles with diameters of 150-250 nm are prepared, and their hydrolytic degradation is studied under physiological conditions. Degradation occurs by hydrolysis of the side chain methyl ester followed by cleavage of the main-chain ester group with methanol and L-malic acid as the final degradation products. No alteration of the cell viability is found after 1 h of incubation, but toxicity increases significantly after 3 d, probably due to the noxious effect of the released methanol. Anticancer drugs temozolomide and doxorubicin are encapsulated in the NPs with 20-40% efficiency, and their release is monitored using in vitro essays. Temozolomide is fully liberated within several hours, whereas doxorubicin is steadily released from the particles over a period of 1 month.
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / pharmacology*
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Cell Death / drug effects
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Cell Line, Tumor
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Cell Survival / drug effects
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Dacarbazine / analogs & derivatives
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Dacarbazine / pharmacology
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Doxorubicin / pharmacology
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Drug Compounding / methods*
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Emulsions
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Humans
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Hydrogen-Ion Concentration / drug effects
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Hydrolysis / drug effects
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Magnetic Resonance Spectroscopy
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Malates / chemical synthesis*
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Malates / chemistry
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Nanoparticles / chemistry*
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Nanoparticles / ultrastructure
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Particle Size
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Polyesters / chemical synthesis*
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Polyesters / chemistry
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Polymers / chemical synthesis*
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Polymers / chemistry
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Powders
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Sonication
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Temozolomide
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Time Factors
Substances
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Antineoplastic Agents
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Emulsions
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Malates
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Polyesters
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Polymers
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Powders
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poly(alpha-methyl-beta-malate)
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poly(malate)
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Dacarbazine
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Doxorubicin
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Temozolomide