A GBS culture collected shortly after GBS prophylaxis may be inaccurate

J Matern Fetal Neonatal Med. 2012 Jun;25(6):736-8. doi: 10.3109/14767058.2011.596961. Epub 2011 Aug 1.

Abstract

Objective: To determine whether a vaginal-rectal culture obtained after antibiotic therapy has begun accurately detects pre-existing colonization with group B streptococcus (GBS).

Methods: A prospective cohort study of women presenting at in labor who were known to be colonized with GBS were recruited. A GBS culture was obtained prior to administration of intravenous penicillin prophylaxis and repeated 2 hours following the first dose of penicillin. The two results were compared.

Results: Eighty subjects were recruited. Complete results were obtained for 61 (76 %) subjects. Of these, 47 (77 %) had a GBS positive on initial culture. Persistence of GBS 2 hours after antibiotic exposure was seen in 30/47 (64 %). Conversion from GBS positive to GBS negative status was seen in 17/47 (36%).

Conclusions: A vaginal-rectal culture for GBS performed after antibiotic prophylaxis has commenced may not accurately reflect a patient's GBS colonization status.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Anti-Bacterial Agents / administration & dosage
  • Antibiotic Prophylaxis* / methods
  • Bacteriological Techniques / methods
  • Bacteriological Techniques / standards
  • Drug Administration Schedule
  • False Negative Reactions
  • Female
  • Humans
  • Penicillins / administration & dosage*
  • Pregnancy
  • Pregnancy Complications, Infectious / microbiology
  • Pregnancy Complications, Infectious / prevention & control*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Specimen Handling* / methods
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / prevention & control*
  • Streptococcus agalactiae / isolation & purification*
  • Time Factors
  • Young Adult

Substances

  • Anti-Bacterial Agents
  • Penicillins