Abstract
Hepatitis C virus (HCV) infects human hepatocytes through several host factors. However, other prerequisite factors for viral entry remain to be identified. Using a yeast two-hybrid screen, we found that human phospholipid scramblase 1 interacts with HCV envelope proteins E1 and E2. These physical interactions were confirmed by co-immunoprecipitation and GST pull-down assays. Knocking down the expression of PLSCR1 inhibited the entry of HCV pseudoparticles. Moreover, PLSCR1 was required for the initial attachment of HCV onto hepatoma cells, where it specifically interacted with entry factor OCLN. We show that PLSCR1 is a novel attachment factor for HCV entry.
Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD / genetics
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Antigens, CD / metabolism
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Cell Line, Tumor
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Claudin-1
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Hepacivirus / metabolism*
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Hepacivirus / physiology
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Humans
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Immunoprecipitation
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Occludin
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Phospholipid Transfer Proteins / genetics
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Phospholipid Transfer Proteins / metabolism*
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Polymerase Chain Reaction
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Protein Binding
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RNA Interference
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Scavenger Receptors, Class B / genetics
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Scavenger Receptors, Class B / metabolism
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Tetraspanin 28
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / metabolism
Substances
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Antigens, CD
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CD81 protein, human
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CLDN1 protein, human
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Claudin-1
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E1 protein, Hepatitis C virus
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Membrane Proteins
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OCLN protein, human
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Occludin
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PLSCR1 protein, human
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Phospholipid Transfer Proteins
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SCARB1 protein, human
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Scavenger Receptors, Class B
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Tetraspanin 28
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Viral Envelope Proteins
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glycoprotein E2, Hepatitis C virus