Sp1 can displace GHF-1 from its distal binding site and stimulate transcription from the growth hormone gene promoter

F P Lemaigre; D A Lafontaine; S J Courtois; S M Durviaux; G G Rousseau

doi:10.1128/mcb.10.4.1811-1814.1990

Sp1 can displace GHF-1 from its distal binding site and stimulate transcription from the growth hormone gene promoter

Mol Cell Biol. 1990 Apr;10(4):1811-4. doi: 10.1128/mcb.10.4.1811-1814.1990.

Authors

F P Lemaigre¹, D A Lafontaine, S J Courtois, S M Durviaux, G G Rousseau

Affiliation

¹ Hormone and Metabolic Research Unit, Louvain University Medical School, Brussels, Belgium.

Abstract

DNase I footprinting experiments showed that binding activities of Sp1 and of GHF-1 to its distal site on the human growth hormone gene promoter are mutually exclusive. The kinetics of GHF-1 binding were indicative of positive cooperativity. The Sp1 site did not affect promoter activity in cell-free transcription. Still, Sp1 could compensate partially for the decreased stimulation of transcription seen at low GHF-1 concentrations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Binding, Competitive
DNA-Binding Proteins / metabolism*
Deoxyribonuclease I
Genes*
Growth Hormone / genetics*
Humans
Kinetics
Molecular Sequence Data
Pituitary Gland, Anterior
Promoter Regions, Genetic*
Restriction Mapping
Sp1 Transcription Factor
Transcription Factors / metabolism*
Transcription, Genetic*

Substances

DNA-Binding Proteins
Sp1 Transcription Factor
Transcription Factors
Growth Hormone
Deoxyribonuclease I

Associated data

GENBANK/M34224