Abstract
The development of convenient, real-time probes for monitoring protein function in biological samples represents an important challenge of the postgenomic era. In response, we introduce here "transcription factor beacons," binding-activated fluorescent DNA probes that signal the presence of specific DNA-binding activities. As a proof of principle, we present beacons for the rapid, sensitive detection of three transcription factors (TATA Binding Protein, Myc-Max, and NF-κB), and measure binding activity directly in crude nuclear extracts.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
MeSH terms
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Base Sequence
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Biosensing Techniques / methods*
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DNA / metabolism
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DNA Probes / metabolism*
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DNA-Binding Proteins
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Fluorescent Dyes / metabolism
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HeLa Cells
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Humans
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Models, Molecular
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Molecular Conformation
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NF-kappa B / analysis
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NF-kappa B / metabolism
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Nanoparticles
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Protein Binding
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Sensitivity and Specificity
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TATA-Box Binding Protein / analysis
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TATA-Box Binding Protein / metabolism
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Transcription Factors / analysis*
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Transcription Factors / metabolism
Substances
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DNA Probes
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DNA-Binding Proteins
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Fluorescent Dyes
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NF-kappa B
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TATA-Box Binding Protein
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Transcription Factors
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DNA