Abstract
Single complex fluorescence polarization spectroscopy is applied to study the peripheral light harvesting antenna (LH2) from photosynthetic purple bacterium Rhodopseudomonas (Rps.) acidophila. The measured two-dimensional excitation-emission polarization plots are used to construct geometric representation for the absorbing B800 and emitting B850 as ellipses. The shape and orientation of the ellipses is discussed in terms of tilted LH2 complexes where emission occurs from energetically disordered B850 excitons.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / chemistry*
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Bacterial Proteins / isolation & purification
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Fluorescence Polarization Immunoassay
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Light-Harvesting Protein Complexes / chemistry*
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Light-Harvesting Protein Complexes / isolation & purification
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Photosynthetic Reaction Center Complex Proteins / chemistry*
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Rhodopseudomonas / chemistry*
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Spectrometry, Fluorescence / methods
Substances
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B800-850 light-harvesting complex, bacteria
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Bacterial Proteins
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Light-Harvesting Protein Complexes
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Photosynthetic Reaction Center Complex Proteins