Abstract
We present a novel denaturing gradient gel electrophoresis (DGGE) method which characterizes multiclonal communities of Staphylococcus aureus. The spa PCR-based DGGE method simultaneously separates strains that differ in only one base, thereby revealing multiclonal colonization and infections.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Coinfection / microbiology*
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DNA, Bacterial / genetics
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Denaturing Gradient Gel Electrophoresis / methods*
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Humans
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Molecular Typing / methods*
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Polymerase Chain Reaction / methods
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Staphylococcal Infections / microbiology*
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Staphylococcal Protein A / genetics*
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Staphylococcus aureus / classification*
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Staphylococcus aureus / genetics*
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Staphylococcus aureus / isolation & purification
Substances
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DNA, Bacterial
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Staphylococcal Protein A