Abstract
A strategy for the site-specific attachment of 2-deoxy-2-fluorosugars to cysteine and dehydroalanine tagged proteins is reported. When combined with thionation of fluorosugars, such as the widely available (18)F probe 2-deoxy-2-[(18)F]fluoroglucose ([(18)F]FDG), this methodology allows fast and direct access to site-specific [(18)F]FDG-labelled proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cysteine / chemistry
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Fluorodeoxyglucose F18 / chemistry*
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Isotope Labeling
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Magnetic Resonance Imaging
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Magnetic Resonance Spectroscopy
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Positron-Emission Tomography
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Proteins / chemistry*
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Radiopharmaceuticals / chemical synthesis
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Radiopharmaceuticals / chemistry*
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Subtilisin / chemistry
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Subtilisin / genetics
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Subtilisin / metabolism
Substances
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Proteins
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Radiopharmaceuticals
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Fluorodeoxyglucose F18
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Subtilisin
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Cysteine