Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells

Sanja Coso; Yiping Zeng; Dhanya Sooraj; Elizabeth D Williams

doi:10.1016/j.yexcr.2011.07.023

Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells

Exp Cell Res. 2011 Oct 15;317(17):2397-407. doi: 10.1016/j.yexcr.2011.07.023. Epub 2011 Aug 2.

Authors

Sanja Coso¹, Yiping Zeng, Dhanya Sooraj, Elizabeth D Williams

Affiliation

¹ Centre for Cancer Research, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia.

PMID: 21839079
DOI: 10.1016/j.yexcr.2011.07.023

Abstract

Lymphatic vessels guide interstitial fluid, modulate immune responses by regulating leukocyte and antigen trafficking to lymph nodes, and in a cancer setting enable tumor cells to track to regional lymph nodes. The aim of the study was to determine whether primary murine lymphatic endothelial cells (mLECs) show conserved vascular endothelial growth factor (VEGF) signaling pathways with human LECs (hLECs). LECs were successfully isolated from murine dermis and prostate. Similar to hLECs, vascular endothelial growth factor (VEGF) family ligands activated MAPK and pAkt intracellular signaling pathways in mLECs. We describe a robust protocol for isolation of mLECs which, by harnessing the power of transgenic and knockout mouse models, will be a useful tool to study how LEC phenotype contributes to alterations in lymphatic vessel formation and function.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Endothelial Cells / metabolism*
Humans
Lymphatic System / cytology*
Mice
Mice, Inbred C57BL
Phenotype
Receptors, Vascular Endothelial Growth Factor / metabolism*
Signal Transduction*

Substances

Receptors, Vascular Endothelial Growth Factor