Evaluation of Salmonella enterica serovar Typhimurium and Choleraesuis slyA mutant strains for use in live attenuated oral vaccines

Takeshi Haneda; Nobuhiko Okada; Yuji Kikuchi; Masami Takagi; Tetsuro Kurotaki; Tsuyoshi Miki; Setsuo Arai; Hirofumi Danbara

doi:10.1016/j.cimid.2011.07.001

Evaluation of Salmonella enterica serovar Typhimurium and Choleraesuis slyA mutant strains for use in live attenuated oral vaccines

Comp Immunol Microbiol Infect Dis. 2011 Sep;34(5):399-409. doi: 10.1016/j.cimid.2011.07.001. Epub 2011 Aug 12.

Authors

Takeshi Haneda¹, Nobuhiko Okada, Yuji Kikuchi, Masami Takagi, Tetsuro Kurotaki, Tsuyoshi Miki, Setsuo Arai, Hirofumi Danbara

Affiliation

¹ Department of Microbiology, School of Pharmacy, Kitasato University, Minato-ku, Tokyo 108-8641, Japan.

PMID: 21840053
DOI: 10.1016/j.cimid.2011.07.001

Abstract

SlyA protein plays a key role in virulence in Salmonella enterica. In this study, we evaluated the ability of the slyA mutant strain of S. enterica serovar Choleraesuis (S. choleraesuis) to protect against swine salmonellosis. Using a murine model infected with S. enterica serovar Typhimurium (S. typhimurium), we showed that the Salmonella strain with a deletion of slyA could be used as a highly immunogenic, effective and safe vaccine in mice. Based on these data, a slyA mutant of S. enterica serovar Choleraesuis strain RF-1 was constructed, and the ability of this mutant to protect immunized pigs from S. choleraesuis infection was examined. As with the S. typhimurium slyA mutant, immunization of pigs with the S. choleraesuis slyA mutant strain provided significant protection against subsequent challenge by the wild-type RF-1. These results demonstrate that SlyA is a potential target in the development of a novel live attenuated vaccine against S. enterica.

Publication types

Randomized Controlled Trial

MeSH terms

Administration, Oral
Animals
Antibody Formation
Bacterial Proteins / genetics*
Disease Models, Animal
Female
Immunity, Humoral
Interferon-alpha / analysis
Interferon-alpha / immunology
Interleukin-4 / analysis
Interleukin-4 / immunology
Lymph Nodes / microbiology
Lymph Nodes / pathology
Mice
Mice, Inbred BALB C
Mutation
Peyer's Patches / microbiology
Peyer's Patches / pathology
Salmonella Infections / immunology*
Salmonella Infections / microbiology
Salmonella Infections / therapy
Salmonella Vaccines / administration & dosage
Salmonella Vaccines / immunology*
Salmonella typhimurium / genetics
Salmonella typhimurium / immunology*
Salmonella typhimurium / pathogenicity
Spleen / microbiology
Spleen / pathology
Swine
Transcription Factors / genetics*
Tumor Necrosis Factor-alpha / analysis
Tumor Necrosis Factor-alpha / immunology
Vaccination
Vaccines, Attenuated / administration & dosage
Vaccines, Attenuated / immunology
Virulence

Substances

Bacterial Proteins
Interferon-alpha
Salmonella Vaccines
Transcription Factors
Tumor Necrosis Factor-alpha
Vaccines, Attenuated
salmolysin
Interleukin-4