Diagnosis of congenital toxoplasmosis by polymerase chain reaction on neonatal peripheral blood

Yvon Sterkers; Jennifer Ribot; Sahar Albaba; Eric Issert; Patrick Bastien; Francine Pratlong

doi:10.1016/j.diagmicrobio.2011.06.006

Diagnosis of congenital toxoplasmosis by polymerase chain reaction on neonatal peripheral blood

Diagn Microbiol Infect Dis. 2011 Oct;71(2):174-6. doi: 10.1016/j.diagmicrobio.2011.06.006.

Authors

Yvon Sterkers¹, Jennifer Ribot, Sahar Albaba, Eric Issert, Patrick Bastien, Francine Pratlong

Affiliation

¹ Department of Parasitology-Mycology, Faculty of Medicine, University Hospital Centre of Montpellier and University Montpellier I, Montpellier, France. [email protected]

PMID: 21856107
DOI: 10.1016/j.diagmicrobio.2011.06.006

Abstract

In a cohort of 12 consecutive neonates, polymerase chain reaction (PCR) established the diagnosis of 5 of 6 cases of congenital toxoplasmosis and did so earlier than serologic methods. We validated that PCR using neonatal peripheral blood is a sensitive, rapid, and cost-effective method to affirm the diagnosis of previously undiagnosed congenital toxoplasmosis.

MeSH terms

Female
Follow-Up Studies
Humans
Infant, Newborn
Infectious Disease Transmission, Vertical
Polymerase Chain Reaction / methods*
Pregnancy
Pregnancy Complications, Parasitic / diagnosis
Prospective Studies
Toxoplasma / isolation & purification
Toxoplasma / pathogenicity
Toxoplasmosis, Congenital / blood*
Toxoplasmosis, Congenital / diagnosis*
Toxoplasmosis, Congenital / parasitology