Abstract
Brain metastasis frequently occurs in cancer patients and is associated with a poor prognosis. We previously reported that S100B was highly expressed in PC14/B, a specific brain metastatic lung adenocarcinoma cell line, which suggests that it is associated with brain metastasis of lung cancer. However, the role of S100B in brain metastasis remains to be elucidated. In this study, using PC14/B cell line, we found that siRNA mediated depletion of S100B in PC14/B cells led to notable differences in cell proliferation, apoptosis, cell cycle progression, colony formation ability, cell migratory and invasive activity compared with the mock-transfected cells. Therefore, our data suggest that S100B promotes the brain metastasis of lung adenocarcinoma by promoting cell proliferation, preventing apoptosis and increasing cell migration and invasion.
Copyright © 2011 John Wiley & Sons, Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Adenocarcinoma / pathology*
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Adenocarcinoma of Lung
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Apoptosis
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Blotting, Western
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Brain Neoplasms / metabolism
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Brain Neoplasms / secondary*
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Bromodeoxyuridine / metabolism
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Cell Line, Tumor
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Cell Movement
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Cell Proliferation
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Gene Expression Regulation, Neoplastic*
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Gene Knockdown Techniques
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Humans
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Lung Neoplasms / genetics
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology*
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Neoplasm Invasiveness / genetics
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Neoplasm Invasiveness / pathology
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Nerve Growth Factors / genetics
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Nerve Growth Factors / metabolism*
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
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S100 Calcium Binding Protein beta Subunit
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S100 Proteins / genetics
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S100 Proteins / metabolism*
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Transfection
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bcl-X Protein / genetics
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bcl-X Protein / metabolism
Substances
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BCL2L1 protein, human
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Nerve Growth Factors
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RNA, Small Interfering
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S100 Calcium Binding Protein beta Subunit
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S100 Proteins
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S100B protein, human
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bcl-X Protein
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Bromodeoxyuridine