One-pot three-enzyme synthesis of UDP-GlcNAc derivatives

Chem Commun (Camb). 2011 Oct 14;47(38):10815-7. doi: 10.1039/c1cc14034e. Epub 2011 Aug 23.

Abstract

A Pasteurella multocida N-acetylglucosamine 1-phosphate uridylyltransferase (PmGlmU) was cloned and used efficiently with an N-acetylhexosamine 1-kinase (NahK_ATCC55813) and an inorganic pyrophosphatase (PmPpA) for one-pot three-enzyme synthesis of UDP-GlcNAc derivatives with or without further chemical diversification.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetylglucosamine / chemical synthesis
  • Acetylglucosamine / chemistry*
  • Bacterial Proteins / metabolism*
  • Bifidobacterium / enzymology
  • Inorganic Pyrophosphatase / metabolism*
  • Nucleotidyltransferases / metabolism*
  • Pasteurella multocida / enzymology
  • Uridine Diphosphate N-Acetylglucosamine / chemical synthesis*
  • Uridine Diphosphate N-Acetylglucosamine / chemistry

Substances

  • Bacterial Proteins
  • Uridine Diphosphate N-Acetylglucosamine
  • Nucleotidyltransferases
  • UDPacetylglucosamine pyrophosphorylase
  • Inorganic Pyrophosphatase
  • Acetylglucosamine