Intrastrand locks increase duplex stability and base pairing selectivity

Cora Prestinari; Clemens Richert

doi:10.1039/c1cc14008f

Intrastrand locks increase duplex stability and base pairing selectivity

Chem Commun (Camb). 2011 Oct 14;47(38):10824-6. doi: 10.1039/c1cc14008f. Epub 2011 Aug 24.

Authors

Cora Prestinari¹, Clemens Richert

Affiliation

¹ Institute for Organic Chemistry, University of Stuttgart, Pfaffenwaldring 55, 70565 Stuttgart, Germany.

PMID: 21863176
DOI: 10.1039/c1cc14008f

Abstract

Oligodeoxynucleotide probes with disulfide locks between neighboring nucleobases show increases in melting point for duplexes with RNA target strands of up to 7.6 °C. The weakly pairing TT dimers are replaced with locked 2'-deoxy-5-(thioalkynyl)uridine residues via automated synthesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Pair Mismatch
Base Pairing
Dimerization
Disulfides / chemistry
Nucleic Acid Hybridization
Oligodeoxyribonucleotides / chemistry*
Oligonucleotide Probes / chemistry
Oligonucleotides / chemistry
RNA / chemistry*
RNA / metabolism
Transition Temperature
Ultraviolet Rays

Substances

Disulfides
Oligodeoxyribonucleotides
Oligonucleotide Probes
Oligonucleotides
locked nucleic acid
RNA