Abstract
The expression of the protooncogenes ETS1 and ETS2 has been studied in purified human T cells activated either by cross-linking of the T-cell receptor-CD3 complex on their cell surface or by direct stimulation with phorbol esters and ionomycin. Our results show that resting T cells express high levels of ETS1 mRNA and protein, while expression of ETS2 is undetectable. Upon T-cell activation, ETS2 mRNA and proteins are induced, while ETS1 gene expression decreases to very low levels. Late after stimulation, ETS1 mRNA is reinduced and maintained at a high level, while ETS2 gene expression decreases to undetectable levels. Therefore, it appears that in human T cells, ETS2 gene products are associated with cellular activation and proliferation, while ETS1 gene products are preferentially expressed in a quiescent state.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Calcium / metabolism
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Cells, Cultured
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DNA Probes
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DNA-Binding Proteins*
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Enzyme Activation
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Gene Expression Regulation* / drug effects
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Genes, Regulator*
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Humans
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Ionomycin / pharmacology
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Lymphocyte Activation*
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Models, Biological
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Phorbol 12,13-Dibutyrate / pharmacology
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Protein Kinase C / metabolism
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Protein-Tyrosine Kinases / genetics
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Protein c-ets-2
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-ets
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Proto-Oncogenes*
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RNA, Messenger / genetics
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Repressor Proteins*
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T-Lymphocytes / drug effects
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T-Lymphocytes / enzymology
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T-Lymphocytes / immunology*
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Tetradecanoylphorbol Acetate / pharmacology
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Trans-Activators*
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Transcription Factors*
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Transcription, Genetic
Substances
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DNA Probes
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DNA-Binding Proteins
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ERF protein, human
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ETS1 protein, human
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ETS2 protein, human
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Protein c-ets-2
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ets
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RNA, Messenger
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Repressor Proteins
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Trans-Activators
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Transcription Factors
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Phorbol 12,13-Dibutyrate
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Ionomycin
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Protein-Tyrosine Kinases
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Protein Kinase C
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Tetradecanoylphorbol Acetate
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Calcium