Analysis of bulk and virus-specific CD8+ T cells reveals advanced differentiation of CD8+ T cells in patients with common variable immunodeficiency

Martin Kuntz; Sigune Goldacker; Hubert E Blum; Hanspeter Pircher; Susanne Stampf; Hans-Hartmut Peter; Robert Thimme; Klaus Warnatz

doi:10.1016/j.clim.2011.07.006

Analysis of bulk and virus-specific CD8+ T cells reveals advanced differentiation of CD8+ T cells in patients with common variable immunodeficiency

Clin Immunol. 2011 Nov;141(2):177-86. doi: 10.1016/j.clim.2011.07.006. Epub 2011 Aug 5.

Authors

Martin Kuntz¹, Sigune Goldacker, Hubert E Blum, Hanspeter Pircher, Susanne Stampf, Hans-Hartmut Peter, Robert Thimme, Klaus Warnatz

Affiliation

¹ Department of Medicine II, University Medical Center Freiburg, Germany.

PMID: 21873117
DOI: 10.1016/j.clim.2011.07.006

Abstract

Common variable immunodeficiency (CVID) is a heterogeneous antibody deficiency syndrome with alterations in T cell regulation and function in a subgroup of patients. We assessed phenotype and function of bulk and virus-specific CD8+ T cells of a cohort of 34 HLA-A2+ CVID patients by pentamer technology and flow cytometry in relationship to their immunological and clinical phenotypes. Bulk CD8+ T cells displayed a shift toward a more antigen experienced and activated differentiation state. The advanced differentiation pattern was mainly found in a subgroup of CVID patients with lymphadenopathy and granulomatous disease. This effect existed independently of the patients' CMV status even so CMV-associated immunosenescence was more evident in CVID patients than in CMV-positive immunocompetent controls. As the phenotype and function of virus-specific CD8+ T cells were normal in CVID the induction of antiviral immunity by prophylactic immunization appears to be a logical and desirable aim for this group of patients.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antigens, Viral / immunology
CD8-Positive T-Lymphocytes / immunology*
Cell Differentiation
Common Variable Immunodeficiency / immunology*
Common Variable Immunodeficiency / pathology
Cytomegalovirus / immunology*
Cytomegalovirus Infections / blood
Cytomegalovirus Infections / immunology
Epstein-Barr Virus Infections / blood
Epstein-Barr Virus Infections / immunology
Female
Granuloma / etiology
Granuloma / immunology
Herpesvirus 4, Human / immunology*
Histocompatibility Antigens Class I / immunology
Humans
Immunodominant Epitopes / immunology
Immunophenotyping
Lymphatic Diseases / etiology
Lymphatic Diseases / immunology
Male
Middle Aged
Oligopeptides / immunology
Orthomyxoviridae / immunology*
Peptide Fragments / immunology
Phosphoproteins / immunology
T-Cell Antigen Receptor Specificity*
T-Lymphocyte Subsets / immunology*
Viral Matrix Proteins / immunology
Viremia / immunology
Young Adult

Substances

Antigens, Viral
Histocompatibility Antigens Class I
Immunodominant Epitopes
Oligopeptides
Peptide Fragments
Phosphoproteins
Viral Matrix Proteins
cytomegalovirus matrix protein 65kDa
glycyl-leucyl-cysteinyl-threonyl-leucyl-valyl-alanyl-methionyl-leucine
influenza matrix peptide (58-66)