Abstract
Glycosynthase mutants of Rhodococcus sp. endo-glycoceramidase II efficiently synthesize complex glycosphingolipids. Glycosyl fluoride donors may be assembled via sequential glycosyltransferase-catalysed glycosylation of lactosyl fluoride. Alternatively, lactosyl fluoride may be coupled to sphingosine prior to subsequent glycosylation steps.
This journal is © The Royal Society of Chemistry 2011
MeSH terms
-
Glycoside Hydrolases / genetics
-
Glycoside Hydrolases / metabolism*
-
Glycosphingolipids / biosynthesis*
-
Glycosphingolipids / chemistry
-
Glycosylation
-
Glycosyltransferases / genetics
-
Glycosyltransferases / metabolism*
-
Lactose / analogs & derivatives
-
Lactose / chemistry
-
Recombinant Proteins / genetics
-
Recombinant Proteins / metabolism
-
Rhodococcus / enzymology
Substances
-
Glycosphingolipids
-
Recombinant Proteins
-
lactosyl fluoride
-
Glycosyltransferases
-
Glycoside Hydrolases
-
endoglycoceramidase
-
Lactose