Genetic diversity of carbapenem-hydrolysing β-lactamases in Acinetobacter baumannii from Romanian hospitals

Clin Microbiol Infect. 2011 Oct;17(10):1524-8. doi: 10.1111/j.1469-0691.2011.03622.x. Epub 2011 Aug 29.

Abstract

Thirteen carbapenem-resistant Acinetobacter baumannii isolates, collected in Romania during 2009-2010, were investigated to identify the mechanism(s) responsible for carbapenem resistance. Genotyping was performed by pulsed-field gel electrophoresis, multiplex PCR sequence typing and multilocus sequence typing. Eleven non-clonally related isolates harboured the bla(OXA-23) gene on their chromosome within a Tn2008 transposon structure. The two remaining isolates harboured a bla(OXA-58) gene that was either plasmid or chromosome borne. Two isolates co-expressed OXA-23 together with the extended-spectrum β-lactamase PER-1. This study constitutes the first report of OXA-58 and OXA-23-producing A. baumannii isolates in Romania.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter Infections / epidemiology
  • Acinetobacter Infections / microbiology
  • Acinetobacter baumannii / drug effects
  • Acinetobacter baumannii / genetics*
  • Acinetobacter baumannii / isolation & purification
  • Acinetobacter baumannii / metabolism
  • Bacterial Typing Techniques
  • Carbapenems / pharmacology
  • Drug Resistance, Bacterial*
  • Electrophoresis, Gel, Pulsed-Field
  • Genetic Variation
  • Genotype
  • Hospitals
  • Humans
  • Microbial Sensitivity Tests
  • Multilocus Sequence Typing
  • Romania / epidemiology
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Carbapenems
  • beta-lactamase OXA-23
  • beta-Lactamases
  • beta-lactamase OXA-58, Acinetobacter baumannii