A graphene oxide-peptide fluorescence sensor tailor-made for simple and sensitive detection of matrix metalloproteinase 2

Duan Feng; Yangyang Zhang; Tingting Feng; Wen Shi; Xiaohua Li; Huimin Ma

doi:10.1039/c1cc13975d

A graphene oxide-peptide fluorescence sensor tailor-made for simple and sensitive detection of matrix metalloproteinase 2

Chem Commun (Camb). 2011 Oct 14;47(38):10680-2. doi: 10.1039/c1cc13975d. Epub 2011 Sep 2.

Authors

Duan Feng¹, Yangyang Zhang, Tingting Feng, Wen Shi, Xiaohua Li, Huimin Ma

Affiliation

¹ Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

PMID: 21892449
DOI: 10.1039/c1cc13975d

Abstract

A graphene oxide-peptide based fluorescence sensor has been developed for matrix metalloproteinase 2 (MMP2), and its applicability has been demonstrated by monitoring the concentration of MMP2 secreted by HeLa cells, revealing that HeLa cells with a density of 5.48 × 10(5) cells per mL can produce 22 nM in cell culture media in 24 h.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorescent Dyes / chemistry
Graphite / chemistry*
HeLa Cells
Humans
Matrix Metalloproteinase 2 / analysis*
Oxides / chemistry*
Peptides / chemistry*
Phenanthrolines / chemistry
Spectrometry, Fluorescence / methods*

Substances

Fluorescent Dyes
Oxides
Peptides
Phenanthrolines
Graphite
Matrix Metalloproteinase 2
1,10-phenanthroline